Prof. Dipl.-Ing. Dr. Winfried Neuhaus

Department of
Pharmaceutical Technology and Biopharmaceutics

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Curriculum vitae

Publications and Posters


My research interests

Our research group

Information for students (in German)

Table soccer (in German)



Biological Barriers in Health and Disease

Welcome to the website of the group of Dr. Neuhaus. We are working on the function of biological barriers in health and disease. The main focus is set on the blood-brain barrier, the lung alveolar epithelium and the kidney proximal tubulus.
In several diseases such as stroke, traumatic brain injury or sepsis the stabilization of biological barriers can contribute to a less adverse outcome and represent a promising therapeutic target.

SUMMARY OF NEWS of last year (2022/2023):
Highlights are summarized here:

- Reviews/books:
Nanotechnological approaches for improved para - and transcellular drug delivery for brain and retinal diseases (Nanoscale Horiz., IF 9.7)

- Topic 3Rs & NAMs:
The rise of 3Rs centres and platforms in Europe (ATLA, IF 2.7)
The current status and work of 3Rs centres and platforms in Europe (ATLA, IF 2.7)
Incorporation of NAMs into regulatory nonclinical pharmaceutical safety assessment (ALTEX, IF 5.6)
Open Letter: Selection of a new Executive Director of the European Chemicals Agency (ECHA) (ALTEX, IF 5.6)

- Topic blood-brain barrier:
CNS pharmacokinetics of novel dopamine transporter inhibitor CE-123 (Int. J. Mol. Sci., IF 5.6)
Subcellular trafficking and transcytosis efficacy of different receptors for antibody delivery across the BBB (FBCNS, IF 7.3)
Influence of cells of the neurovascular unit in an hiPSC- based late onset Alzheimer's disease model (
FBCNS, IF 7.3)
A hydrogel BBB in vitro model based on hiPSCs (PLoS One, IF 3.7)

Activation of non-classical NMDARs by glycine impairs function of brain endothelial cells (Cell Mol. Life Sci., IF 8.0)

- Topic biological barriers:
Nanoparticle toxicity in mucosa models to study the uptake via the air pathway is dependent on the cultivation modality and barrier maturity (Int. J. Mol. Sci., IF 5.6)
A primary cell based model of the human small intestine reveals host olfactomedin 4 induction in response to Salmonella Typhimurium infection (Gut microbes, IF 12.2)
A human stem-cell derived brain-liver chip for assessment of BBB drug permeation (Cells, IF 6.0)

APPOINTMENT to PROFESSOR FOR 3Rs AND NAMs (New approach methodologies), October 2022, here some collected media reports:

ORF online, 3.10.2022:

Privatuni Krems: Erste Professur für Alternativen zum Tierversuch - science.ORF.at

NÖN, 3.10.2022:
"Starker neuer Impuls" - Erste Professur für Alternativen zum Tierversuch an DPU Krems - NÖN.at (noen.at)

Salzburger Nachrichten, 3.10.2022:

Erste Professur für Alternativen zum Tierversuch an DPU Krems | SN.at

Heute, 3.10.2022:

Statt Tierversuchen – Privatuni sucht nun Alternativen – Niederösterreich | heute.at

Kurier, 5.10.2022:

Erste Professur für Alternativen zum Tierversuch an DPU Krems | kurier.at

Kronen Zeitung, 8.10.2022:

An Kremser Privat-Uni - Alternativen zu Tierversuchen werden nun erforscht | krone.at

Salzburger Nachrichten, 22.11.2022:

Professor sucht Alternativen zu Tierversuchen | SN.at

SUMMARY OF NEWS of last years (2020-2022):
About 20 contributions have been published, the highlights are summarized here:

- Reviews/books
Review about Extracellular vesicles as cancer biomarkers (Adv. Drug Delivery Revs, IF 15.47)
Book chapter about Blood-brain barrier in vitro models (Handb Exp Pharmcol)
Book chapter about Guidelines about transport studies across BBB models (Handb Exp Pharmacol)
Book Physiology, Pharmacology and Pathology of the Blood-Brain Barrier (Springer)

- Topic blood-brain barrier
A Microfluidic Multisize Spheroid Array for Blood-Brain Barrier Screening (Adv Science, IF 15.44)
The pivotal role of microenvironmental cells in stroke blood-brain barrier breakdown (FBCNS, IF 7.67)
SARS-CoV2 dysregulates the blood-brain barrier and might enter the CNS (Stem Cell Rep, IF  7.75)
TAT-MeCP2 as replacement therapy for RETT syndrome (Int J Biol Macromol, IF 6.95)

- Topic blood-saliva barrier
Development of salivary gland epithelial cell model for biomarker studies (Biomedicines, IF 6.08)
CRP as salivary biomarker for neonatal sepsis (Pharmaceutics, IF 6.32)

Optimization of an oral mucosa model (Tissue Barrier)
NEWS: "Die Krone" article "Medizin fürs Gehirn" in the newspaper with the largest readership in Austria, 26.11.2019 about our work at AIT.

New Future Science OA Paper about an autologous 4-organ-on-a-chip system based on human, induced pluripotent stem cells (hiPSCs).

NEWS: Article "Am biochemischen Türsteher vorbeikommen" in Austrian Newspaper "DiePresse" about our work.

NEWS: New Nature Scientific Reports Paper (IF 4.01) about the development of an ECLIA assay to measure MeCP2 and application in research about the Rare Disease RETT syndrome

NEWS: New Annals of Neurology (IF 10.25) Paper about the pivotal role of PAI-1 during fibrinolysis in traumatic brain injury

NEWS: New ACS Appl Mater Interfaces (IF 8.09) Paper about a novel material to control oxygen levels at the microscale for e.g. a microfluidic in vitro stroke model of the blood-brain barrier

NEWS: New Biomaterials (IF 8.81) Paper about a novel tool to transiently open the  blood-brain barrier for drug delivery of small compounds

NEWS: New Tissue Barriers Review about cell culture models of the blood-saliva barrier.

NEWS: Article in Austrian Newspaper "Die Krone" about our Alternatives to Animal Testing efforts at EUSAAT (www.eusaat.org)

NEWS: New Frontier Molecular Neuroscience Paper (IF 5.08) about the compound WY-14643 to reduce traumatic brain injury damage.

NEWS: New Stem Cell Reports Paper (IF 7.34) about a novel human blood-brain barrier model based on human induced, pluripotent stem cells (hiPSCs).

NEWS: I am looking forward to my new position at the Austrian Institute of Technology (AIT, www.ait.ac.at)  starting in April 2016.

NEWS: Link to the article on the website of the University of Vienna: "Risiken und Nebenwirkungen in der Behandlung Frühgeborener", went online on 9th of February 2016.

NEWS: New PlosONE Paper about "Balanced hydroxyethylstarch (HES 130/0.4) impairs kidney function in-vivo without inflammation" went online on 5th of September 2015.

New PlosONE Paper about "Multiple antenatal dexamethasone treatment alters brain vessel differentation in newborn mouse pups" went online on 14th of August 2015.

NEWS: Winfried Neuhaus is an Invited Speaker at the "2nd Zing Barriers of The CNS Conference" from 27th to 30th September 2015 in Oropesa, Spain, and will talk about ischemia in-vitro models, if interested in the conference follow the link: http://www.zingconferences.com/conferences/2nd-zing-barriers-cns-conference/

NEWS: Link to the video and article on the website of the University of Vienna: "Das Hirn und sein Hüter"

Cldn5Light microscope BMECs

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