Soft-tissue imaging for comparative embryology, functional morphology, systematics, and morphometric studies... Metscher 2009, BMC Physiol 9:11.
(Click on "Publications" in the left column for more)
...and developmental morphology of model and non-model species with histological detail in 3D.
We are currently working to update and streamline techniques for 3D imaging of molecular probes in whole samples.
Chick embryo immunostained for acetylated α-tubulin, contrasted with peroxidase-mediated reduced silver deposition. Metscher and Müller 2011, Dev Dyn 240:2301.
Another ongoing project is developing methods for simultaneous imaging of different materials within a sample.
Double-stained cat toe pad with different tissues distinguished by dual-energy image decomposition. Handschuh et al. 2017, J. Microscopy 267:3-26.
...like the millipede Ommatoiulus avatar. High-detail
3D images of irreplaceable specimens can be freely shared as a
resource for systematics and biodiversity research.
Akkari et al. 2015, PLoS ONE 10:8.
Akkari et
al. 2018, PLoS ONE 13:7.
Canine skin lesion hemisected vertically. Virtual sections near the common (cut) surface (9µm voxels). L: Lugol staining, favours keratin, adipose tissue. R: PTA staining - favours collagen, connective tissue. Collaboration with Dr. R. Doughty, AHUS, Oslo.