Assistants:
Mag. Petra Pokorny
petra.pokorny@univie.ac.at
Anne Petrasko
DieAnne@gmx.at
Class time: Wednesdays, 12:30
s.t. - 15h, in the Theoretical Biology Imaging Lab (Ebene 2,
Spange 3).
The first class meeting will be on 10 October.
Structure of the course:
We will meet once per week, in the Theoretical Biology Department
seminar room or in the Imaging Lab. The course is really a
combination of lab, lecture, and seminar: attendance and
participation are essential. There will also be some homework
assignments, demonstrations and simulations, and small
projects. The emphasis throughout will be on conceptual and
practical understanding of imaging and visualisation processes and
methods.
Readings will be assigned from various articles, web pages, etc.
Most will be posted on this page.
This course is a prerequisite for the Summer Semester Project
Practicum on microCT (300416).
Evaluation:
This is a Prüfungsimmanent
course, i.e. no exams. During the course, I will give you problems
and exercises, some of which we will discuss in class and some of
which will be graded as homework. Your final mark will depend
mainly on attendance and participation throughout the semester.
Course Topics:
Topics can be adjusted somewhat according to students' interests.
How we see: the human visual system
Optical imaging based on refraction
Light microscopy: how it works; different kinds of microscopes
Diffraction by edges, gratings
Image quality and imaging system performance
Light microscopy revisited: diffraction-based description
Concepts of resolution
What limits resolution in different systems
Digital imaging
Manipulating and processing digital image files
Projection imaging: EM and x-ray imaging
From 2-D to 3-D: why we need three spatial dimensions, and how we
can visualise them
Tomographic imaging basics
MRI, OPT, episcopic, and other 3-D modalities (PET, SPECT)
Micro-CT theory, technology, and applications
Approximately half of the semester will be devoted to
3-dimensional imaging, mainly x-ray microtomography
(microCT). In-vivo imaging, clinical applications, and
electron microscopy will not be covered in any detail.
Resources: